polya rna enrichment Search Results


polya rna enrichment  (Azenta)
86
Azenta polya rna enrichment
SEN1 (NH2473) and sen1-md (NH2520) were transferred to Spo medium and cells were taken at various timepoints. (A) Meiotic progression. Fixed cells were stained with DAPI and examined by fluorescence microscopy for the presence of MI and MII cells. (B) <t>RNA-seq</t> analysis. <t>mRNA</t> was isolated at the indicated timepoints from the timecourse shown in Panel A. Numbers above each column indicate hours after transfer to Spo medium. The z value for each timepoint ( n ) was calculated as [(FPKM n -FPKM ave )/SD], where FPKM = fragments per kilobase per million, SD = standard deviation and ave = average. Genes were sorted based on the RNA expression patterns in the SEN1 diploid by clustering analysis (k-means) using Cluster 3.0. The heat map was generated using Java TreeView. Green indicates z values < 0, while red indicates z values > 0. (C) A subset of previously identified IME1 -dependent early genes and NDT80 -dependent middle genes were selected and clustered as in Panel B. Gene names in orange encode proteins that were examined in , while IME1 is written in blue. (D) Meiotic progression in P CUP1 -IME1 diploids. Diploids containing P CUP1 -IME1 (39807; n = 4), P CUP1 -IME1 sen1-md (NH2842; n = 5) or P CUP1 -IME1 sen1-md::P REC8 -SEN1 (NH2842::pBG45; n = 5) were incubated in Spo medium for 2 hours at which time CuSO 4 was added to a final concentration of 50 μM to induce IME1 expression. Cells at the indicated timepoints were stained with DAPI and meiotic progression analyzed as in . The 0 hour timepoint is the time of copper addition. The average values for each timepoint were graphed. (E) T 50 analysis of the timecourses from Panel D. The T 50 value indicates the time it took after the addition of copper at which the half-maximal %MI + MII value was reached. Data were analyzed for statistical significance using an unpaired, two-tailed Student’s t-test. **** = p < 0.0001.
Polya Rna Enrichment, supplied by Azenta, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/polya+rna+enrichment/pmc12714266-406-13-8?v=Azenta
Average 86 stars, based on 1 article reviews
polya rna enrichment - by Bioz Stars, 2026-07
86/100 stars
  Buy from Supplier

Image Search Results


SEN1 (NH2473) and sen1-md (NH2520) were transferred to Spo medium and cells were taken at various timepoints. (A) Meiotic progression. Fixed cells were stained with DAPI and examined by fluorescence microscopy for the presence of MI and MII cells. (B) RNA-seq analysis. mRNA was isolated at the indicated timepoints from the timecourse shown in Panel A. Numbers above each column indicate hours after transfer to Spo medium. The z value for each timepoint ( n ) was calculated as [(FPKM n -FPKM ave )/SD], where FPKM = fragments per kilobase per million, SD = standard deviation and ave = average. Genes were sorted based on the RNA expression patterns in the SEN1 diploid by clustering analysis (k-means) using Cluster 3.0. The heat map was generated using Java TreeView. Green indicates z values < 0, while red indicates z values > 0. (C) A subset of previously identified IME1 -dependent early genes and NDT80 -dependent middle genes were selected and clustered as in Panel B. Gene names in orange encode proteins that were examined in , while IME1 is written in blue. (D) Meiotic progression in P CUP1 -IME1 diploids. Diploids containing P CUP1 -IME1 (39807; n = 4), P CUP1 -IME1 sen1-md (NH2842; n = 5) or P CUP1 -IME1 sen1-md::P REC8 -SEN1 (NH2842::pBG45; n = 5) were incubated in Spo medium for 2 hours at which time CuSO 4 was added to a final concentration of 50 μM to induce IME1 expression. Cells at the indicated timepoints were stained with DAPI and meiotic progression analyzed as in . The 0 hour timepoint is the time of copper addition. The average values for each timepoint were graphed. (E) T 50 analysis of the timecourses from Panel D. The T 50 value indicates the time it took after the addition of copper at which the half-maximal %MI + MII value was reached. Data were analyzed for statistical significance using an unpaired, two-tailed Student’s t-test. **** = p < 0.0001.

Journal: PLOS Genetics

Article Title: The conserved SEN1 DNA/RNA helicase has multiple functions during yeast meiosis

doi: 10.1371/journal.pgen.1011684

Figure Lengend Snippet: SEN1 (NH2473) and sen1-md (NH2520) were transferred to Spo medium and cells were taken at various timepoints. (A) Meiotic progression. Fixed cells were stained with DAPI and examined by fluorescence microscopy for the presence of MI and MII cells. (B) RNA-seq analysis. mRNA was isolated at the indicated timepoints from the timecourse shown in Panel A. Numbers above each column indicate hours after transfer to Spo medium. The z value for each timepoint ( n ) was calculated as [(FPKM n -FPKM ave )/SD], where FPKM = fragments per kilobase per million, SD = standard deviation and ave = average. Genes were sorted based on the RNA expression patterns in the SEN1 diploid by clustering analysis (k-means) using Cluster 3.0. The heat map was generated using Java TreeView. Green indicates z values < 0, while red indicates z values > 0. (C) A subset of previously identified IME1 -dependent early genes and NDT80 -dependent middle genes were selected and clustered as in Panel B. Gene names in orange encode proteins that were examined in , while IME1 is written in blue. (D) Meiotic progression in P CUP1 -IME1 diploids. Diploids containing P CUP1 -IME1 (39807; n = 4), P CUP1 -IME1 sen1-md (NH2842; n = 5) or P CUP1 -IME1 sen1-md::P REC8 -SEN1 (NH2842::pBG45; n = 5) were incubated in Spo medium for 2 hours at which time CuSO 4 was added to a final concentration of 50 μM to induce IME1 expression. Cells at the indicated timepoints were stained with DAPI and meiotic progression analyzed as in . The 0 hour timepoint is the time of copper addition. The average values for each timepoint were graphed. (E) T 50 analysis of the timecourses from Panel D. The T 50 value indicates the time it took after the addition of copper at which the half-maximal %MI + MII value was reached. Data were analyzed for statistical significance using an unpaired, two-tailed Student’s t-test. **** = p < 0.0001.

Article Snippet: The cell pellets were sent to GENEWIZ (now Azenta) for RNA extraction and polyA RNA enrichment.

Techniques: Staining, Fluorescence, Microscopy, RNA Sequencing, Isolation, Standard Deviation, RNA Expression, Generated, Incubation, Concentration Assay, Expressing, Two Tailed Test